Classical and Basal Cell Culture Media

A variety of nutrient-rich solutions, including DMEM, RPMI, MEM, etc., used for maintaining and cultivating cells in laboratory settings, cell culture media plays a critical role in cell biology research, providing the necessary environment for cells to thrive and be studied in controlled laboratory conditions.

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Gibco™ Medium 199, Earle's Salts

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.

Pricing & Availability
Specifications

Sterilization Method	Sterile-filtered
Product Type	Medium 199
Form	Liquid
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1X
Sterility	Sterile-filtered
Product Line	Gibco

Gibco™ MEM, HEPES, no glutamine

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.

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Gibco™ BGJb Medium

BGJb medium was developed for the growth of fetal rat long bones in a chemically defined medium.

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Gibco™ AmnioMAX™ C-100 Basal Medium

Part of kit used to prepare fully-supplemented Gibco AmnioMAX C-100 Complete Medium developed for short term culture of human amniotic fluid cells for cytogenetic studies and in vitro diagnostic procedures

Pricing & Availability
Specifications

Sterilization Method	Sterile-filtered
Form	Liquid
Sterility	Sterile-filtered

Gibco™ Waymouth's Medium

Waymouth's is a chemically defined medium developed by Charity Waymouth and originally formulated for studies on the nutrition, metabolism, and growth of mouse Strain L sublines, NCTC clone 929 (L929 cells) in a serum-free environment.

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Gibco™ PSC Cardiomyocyte Maintenance Medium

PSC Cardiomyocyte Maintenance Medium

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Gibco™ CD 293 AGT™ Medium

CD 293 AGT™ is a protein-free, chemically-defined medium in an easy-to-use granular format.

Pricing & Availability
Specifications

Cell Line	293 (HEK)
Included Antibiotics	No antibiotics
Product Type	CD 293 Medium
Form	Powder (AGT)
Culture Type	Suspension Cell Culture
Serum Level	Serum-free

Gibco™ CD BHK-21 Production Medium

CD BHK-21 Production Medium

Pricing & Availability
Specifications

Cell Line	BHK-21
Content And Storage	Store in refrigerator (2–8°C).
Product Type	Production Medium
Form	Powder

Gibco™ Liver Digest Medium

Liver Digest Medium

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100

Gibco™ Medium 199, Earle's Salts, powder

Medium 199 was originally developed for nutritional studies of chick embryo fibroblasts. It has broad species applicability, particularly for cultivation of non-transformed cells.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	Rat epithelial
Content And Storage	Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture
Form	Powder
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Product Line	Gibco
Cell Type	Chick Embryo Fibroblasts, Mouse Pancreatic Epithelium, Rat Lens Tissues

101

Gibco™ DMEM, high glucose, GlutaMAX™ Supplement, pyruvate

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.

Pricing & Availability
Specifications

Sterilization Method	Sterile-filtered
With Additives	High Glucose,GlutaMAX,Phenol Red,Sodium Pyruvate
Form	Liquid
Without Additives	No HEPES
Classification	Animal Origin-free
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1X
Sterility	Sterile-filtered

102

Gibco™ MEM, HEPES, GlutaMAX™ Supplement

Minimum Essential Medium is commonly used of all cell culture media

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Content And Storage	Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Form	Liquid
Without Additives	No Sodium Pyruvate
Concentration	1 X
Sterility	Sterile-filtered
Cell Type	Primary Rat Astrocytes
With Additives	Low Glucose,GlutaMAX,HEPES,Phenol Red
Product Type	MEM (Minimum Essential Medium)
Classification	Animal Origin-free
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco, GlutaMAX
Serum Level	Standard Serum Supplementation

103

Gibco™ MEM, GlutaMAX™ Supplement

Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.

Pricing & Availability
Specifications

Shipping Condition	Room Temperature
Cell Line	HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Content And Storage	Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Form	Liquid
Without Additives	No HEPES,No Sodium Pyruvate
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1 X
Sterility	Sterile-filtered
Cell Type	Primary Rat Astrocytes
Sterilization Method	Sterile-filtered
With Additives	Low Glucose,GlutaMAX,Phenol Red
Product Type	MEM (Minimum Essential Medium)
Classification	Animal Origin-free
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco, GlutaMAX
Serum Level	Standard Serum Supplementation

104

Gibco™ MEM α, nucleosides, no phenol red

Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells.

Pricing & Availability
Specifications

Shipping Condition	Ambient
Cell Line	HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts
Content And Storage	Storage conditions: 2°C to 8°C (protect from light) Shipping conditions: Ambient Shelf life: 12 months from date of manufacture
Form	Liquid
Without Additives	No HEPES,No Phenol Red
Manufacturing Quality	cGMP-compliant under the ISO 13485 standard
Concentration	1X
Sterility	Sterile-filtered
Cell Type	Mammalian Cells
Sterilization Method	Sterile-filtered
With Additives	Low Glucose,Glutamine,Sodium Pyruvate,Ribonucleosides,Deoxyribonucleosides
Product Type	MEM α (Minimum Essential Medium α)
Classification	Animal Origin-free
Shelf Life	12 Months From Date of Manufacture
Product Line	Gibco
Serum Level	Standard Serum Supplementation

105

Gibco™ MEM (Temin's modification) (2X), no phenol red

Temin's Modified Eagle Medium (MEM), is a modified form of Minimum Essential Medium, one of the most commonly used of all cell culture media.

Pricing & Availability

Classical and Basal Cell Culture Media

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Gibco™ Medium 199, Earle's Salts Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ MEM, HEPES, no glutamine Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ BGJb Medium Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ AmnioMAX™ C-100 Basal Medium Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Waymouth's Medium Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ PSC Cardiomyocyte Maintenance Medium Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ Liver Digest Medium Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ DMEM, high glucose, GlutaMAX™ Supplement, pyruvate Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ MEM, HEPES, GlutaMAX™ Supplement Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ MEM, GlutaMAX™ Supplement Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ MEM α, nucleosides, no phenol red Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Gibco™ MEM (Temin's modification) (2X), no phenol red Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More