
Classical and Basal Cell Culture Media














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Gibco™ DMEM, high glucose, no glutamine
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DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.


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Shipping Condition | Room Temperature |
---|---|
Cell Line | HeLa, 293, Cos-7, and PC-12 |
Form | Liquid |
Without Additives | No Glutamine,No HEPES,No Sodium Pyruvate |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Concentration | 1 X |
Sterility | Sterile-filtered |
Cell Type | Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells |
Sterilization Method | Sterile-filtered |
With Additives | High Glucose,Phenol Red |
Product Type | DMEM (Dulbecco's Modified Eagle Medium) |
Classification | Animal Origin-free |
Shelf Life | 12 Months From Date of Manufacture |
Product Line | Gibco™ |
Serum Level | Standard Serum Supplementation |
Gibco™ RPMI 1640 Medium
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer.


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Shipping Condition | Room Temperature |
---|---|
Cell Line | HeLa, Jurkat, MCF-7, PC-12, PBMC, astrocytes, and carcinomas |
Form | Liquid |
Without Additives | No HEPES,No Sodium Pyruvate |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Concentration | 1 X |
Sterility | Sterile-filtered |
Cell Type | Leukemic Cells |
Sterilization Method | Sterile-filtered |
With Additives | Glutamine,Phenol Red |
Product Type | RPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium) |
Classification | Animal Origin-free |
Shelf Life | 12 Months From Date of Manufacture |
Product Line | Gibco™ |
Serum Level | Standard Serum Supplementation |
Gibco™ Ham's F-12 Nutrient Mix, powder
Ham's F-12 Nutrient Mixture (F-12) was designed for serum-free single-cell plating of Chinese Hamster Ovary (CHO) cells.

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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Room Temperature |
---|---|
Content And Storage | Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture |
With Additives | Glutamine,Phenol Red,Sodium Pyruvate |
Product Type | Ham's F-12 Nutrient Mixture |
Form | Powder |
Without Additives | No HEPES,No Sodium Bicarbonate |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Product Line | Gibco™ |
Cell Type | Primary Rat Astrocytes |
Gibco™ Glasgow's MEM, powder
Originally developed by Ian McPherson and Michael Stoker as modification of Eagle's medium

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Gibco™ Opti-MEM™ I Reduced Serum Medium
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Opti-MEM™ I Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change in growth rate or morphology.


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Shipping Condition | Room Temperature |
---|---|
Cell Line | Sp2, AE-1, CHO, BHK-21, and HEK |
Content And Storage | Store at 2–8°C and protect from light. |
Form | Liquid |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Concentration | 1 X |
Sterility | Sterile-filtered |
Cell Type | Primary Fibroblasts |
Sterilization Method | Sterile-filtered |
With Additives | Glutamine,Phenol Red |
Product Type | Opti-MEM I |
Classification | Human Origin |
Shelf Life | 18 Months From Date of Manufacture |
Product Line | Gibco™, Opti-MEM™ |
Gibco™ MEM, HEPES, GlutaMAX™ Supplement
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Minimum Essential Medium is commonly used of all cell culture media


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Shipping Condition | Room Temperature |
---|---|
Cell Line | HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts |
Content And Storage | Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
Form | Liquid |
Without Additives | No Sodium Pyruvate |
Concentration | 1 X |
Sterility | Sterile-filtered |
Cell Type | Primary Rat Astrocytes |
With Additives | Low Glucose,GlutaMAX,HEPES,Phenol Red |
Product Type | MEM (Minimum Essential Medium) |
Classification | Animal Origin-free |
Shelf Life | 12 Months From Date of Manufacture |
Product Line | Gibco™, GlutaMAX™ |
Serum Level | Standard Serum Supplementation |
Gibco™ MEM α, nucleosides, no phenol red
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Minimum Essential Medium (MEM) α is widely used for mammalian cell culture as well as selection for transfected DHFR negative cells.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Room Temperature |
---|---|
Cell Line | HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts |
Content And Storage | Storage conditions: 2-8° C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
Form | Liquid |
Without Additives | No HEPES,No Phenol Red |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Concentration | 1 X |
Sterility | Sterile-filtered |
Cell Type | Primary Rat Astrocytes |
Sterilization Method | Sterile-filtered |
With Additives | Low Glucose,Glutamine,Sodium Pyruvate,Ribonucleosides,Deoxyribonucleosides |
Product Type | MEM α (Minimum Essential Medium α) |
Classification | Animal Origin-free |
Shelf Life | 12 Months From Date of Manufacture |
Product Line | Gibco™ |
Serum Level | Standard Serum Supplementation |
Gibco™ Opti-MEM™ Reduced Serum Medium, powder
Opti-MEM™ Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Room Temperature |
---|---|
Cell Line | Sp2, AE-1, CHO, BHK-21, and HEK |
Content And Storage | Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 30 months from date of manufacture |
With Additives | Glutamine,Phenol Red |
Product Type | Opti-MEM I |
Form | Powder |
Classification | Animal Origin |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Shelf Life | 30 Months From Date of Manufacture |
Sterility | Sterile-filtered |
Product Line | Gibco™, Opti-MEM™ |
Cell Type | Primary Fibroblasts |
Gibco™ MEM, powder
Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.

Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Room Temperature |
---|---|
Cell Line | HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts |
Content And Storage | Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture |
Form | Powder |
Without Additives | No HEPES,No Sodium Pyruvate,No Sodium Bicarbonate |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Sterility | Sterile-filtered |
Cell Type | Primary Rat Astrocytes |
With Additives | Low Glucose,Glutamine,Phenol Red |
Product Type | MEM (Minimum Essential Medium) |
Classification | Animal Origin-free |
Shelf Life | 36 Months From Date of Manufacture |
Product Line | Gibco™ |
Serum Level | Standard Serum Supplementation |
Gibco™ FreeStyle™ F17 Expression Medium
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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An animal origin-free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F and CHO cells in suspension without adaptation.


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Cell Line | HEK293 |
---|---|
Culture Environment | CO2 |
Purity or Quality Grade | Qualified for Further Manufacturing |
Form | Liquid |
Without Additives | No Glutamine |
Endotoxin Level | < 1 EU/mL |
Concentration | 1 X |
For Use With (Application) | Protein expression, viral vector production |
Sterility | Sterile |
Product Type | Expression Medium |
Classification | Animal Origin-free,Chemically-defined,Protein-free,Serum-free |
Culture Type | Suspension Cell Culture |
Product Line | Freestyle™ |
Serum Level | Serum-free |
Gibco™ MEM, high glucose, high sodium bicarbonate
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Gibco™ SILAC RPMI 1640 Flex Media, no glucose, no phenol red
SILAC RPMI 1640 Flex Media, no glucose, no phenol red


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Gibco™ BenchStable™ MEM
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Gibco Minimum Essential Medium (MEM) is one of the most commonly used of all cell culture media.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Room Temperature |
---|---|
Cell Line | HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts |
Content And Storage | Store at room temperature. Protect from light. |
Form | Liquid |
Without Additives | No HEPES,No Sodium Pyruvate |
Manufacturing Quality | cGMP-compliant under the ISO 9001: 2015 standard |
Green Features | Energy efficient, Fewer resources used, Sustainable packaging |
Concentration | 1 X |
Sterility | Sterile-filtered |
With Additives | Low Glucose,GlutaMAX,Phenol Red |
Product Type | MEM (Minimum Essential Medium) |
Classification | Animal Origin-free |
Shelf Life | 12 Months From Date of Manufacture |
Product Line | BenchStable™ |
Serum Level | Standard Serum Supplementation |
Gibco™ DMEM, powder, high glucose, pyruvate
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Shipping Condition | Room Temperature |
---|---|
Cell Line | HeLa, 293, Cos-7, and PC-12 |
Content And Storage | Storage conditions: 2-8° C Shipping conditions: Ambient Shelf life: 36 months from date of manufacture |
Form | Powder |
Without Additives | No HEPES,No Sodium Bicarbonate |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
Sterility | Sterile-filtered |
Cell Type | Primary Fibroblasts, Neurons, Glial Cells, HUVECs, Smooth Muscle Cells |
With Additives | High Glucose,Glutamine,Phenol Red,Sodium Pyruvate |
Product Type | DMEM (Dulbecco's Modified Eagle Medium) |
Classification | Animal Origin-free |
Shelf Life | 36 Months From Date of Manufacture |
Product Line | Gibco™ |
Serum Level | Standard Serum Supplementation |
Gibco™ LHC Basal Medium (1X)
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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LHC Basal Medium (1X)


Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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Product Type | LHC Basal Medium |
---|---|
Form | Liquid |
Classification | Serum-free |
Concentration | 1 X |
Sterility | Sterile-filtered |